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Chapitres
- 00:05Introduction
- 01:14Cyrosectioning
- 01:54Fluorescence Immunolabelling
- 03:55Fluorescence Image Acquisition and Automated Fluorescence Image Analysis
- 04:55Data Analysis
- 06:24Results: Representative Murine Hepatocyte Nuclear Ploidy Estimation
- 08:03Conclusion
We present a robust, cost-effective, and flexible method for measuring changes in hepatocyte number and nuclear ploidy within fixed/cryopreserved tissue samples that does not require flow cytometry. Our approach provides a powerful sample-wide signature of liver cytology ideal for tracking the progression of liver injury and disease.
