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Médecine
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Immunofluorescence Labelling of Human and Murine Neutrophil Extracellular Traps in Paraffin-Embedded Tissue
Journal JoVE
Médecine
This content is Free Access.
Journal JoVE Médecine
Immunofluorescence Labelling of Human and Murine Neutrophil Extracellular Traps in Paraffin-Embedded Tissue

Immunofluorescence Labelling of Human and Murine Neutrophil Extracellular Traps in Paraffin-Embedded Tissue

DOI:
10.3791/60115-v

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07:17 min

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September 10, 2019

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,
1Microscopy Core Facility,Max Planck Institute for Infection Biology, 2Cellular Microbiology,Max Planck Institute for Infection Biology

Chapitres

  • 00:04Titre
  • 00:52Rehydration, Heat-induced Epitope Retrieval, Staining, Mounting, and Microscopic Analysis
  • 03:57Results: Immunofluorescence Labelling of Neutrophil Extracellular Traps in Paraffin-Embedded Tissue
  • 06:16Conclusion

Summary

Traduction automatique

Traduction automatique

Neutrophil extracellular traps (NETs) are three-dimensional structures generated by stimulated neutrophil granulocytes. It has become clear in recent years that NETs are involved in a wide variety of diseases. Detection of NETs in tissue may have diagnostic relevance, so standardized protocols for labelling NET components are required.

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