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Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment
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Neurosciences
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Journal JoVE Neurosciences
Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment

DOI:
10.3791/60118-v

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10:09 min

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June 02, 2020

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, , , ,
1U1192-Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM),Univ. Lille, INSERM

Chapitres

  • 00:05Titre
  • 01:01Isolation of EVs
  • 02:54Characterization of EVs
  • 07:07Results: Analysis of Isolated Extracellular Vesicles
  • 09:16Conclusion

Summary

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The present report highlights chronological requirements for extracellular vesicle (EV) isolation from microglia or blood macrophages. Microglia-derived EVs were evaluated as regulators of the neurite outgrowth while blood macrophage-derived EVs were studied in the control of C6 glioma cell invasion in in vitro assays. The goal is to better understand these EV functions as immune mediators in specific microenvironments.

Tags

Extracellular VesiclesEV IsolationSize Exclusion ChromatographyNanoparticle Tracking AnalysisMass SpectrometryMicrogliaMacrophageCell CultureCharacterization

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