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En 3D spheroid modell som en mer fysiologisk system for kreft-Associated fibroblaster differensiering og Invasion in vitro studier
Journal JoVE
Recherche en cancérologie
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Journal JoVE Recherche en cancérologie
A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies
DOI:

06:27 min

August 08, 2019

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Chapitres

  • 00:04Titre
  • 00:413D Spheroids as an In Vitro Model for Fibroblast/CAF Differentiation Using Different TGF-β1 Inhibiting Compounds of Cell-matrix Interaction
  • 02:02Immunofluorescent Staining of Spheroids
  • 04:44Results: A 3D Spheroid Model for Cancer-Associated Fibroblasts Differentiation and Invasion
  • 05:37Conclusion

Summary

Traduction automatique

Målet med denne protokollen er å etablere en 3D in vitro-modell for å studere differensiering av kreft-assosiert fibroblaster (CAFs) i en svulst bulk-lignende miljø, som kan håndteres i ulike analyse systemer, for eksempel immunofluorescence, transcriptional analyse og livs celle avbildning.

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