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Biaxial Basal Tone and Passive Testing of the Murine Reproductive System Using a Pressure Myograph
Journal JoVE
Bio-ingénierie
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Journal JoVE Bio-ingénierie
Biaxial Basal Tone and Passive Testing of the Murine Reproductive System Using a Pressure Myograph
DOI:

09:59 min

August 13, 2019

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Chapitres

  • 00:04Titre
  • 00:59Reproductive System Dissection and Cannulation
  • 02:15Basal Tone Mechanical Testing
  • 05:55Passive Mechanical Testing
  • 07:54Results: Representative Biaxial Basal Tone and Passive Murine Reproductive System Testing
  • 09:06Conclusion

Summary

Traduction automatique

This protocol utilized a commercially available pressure myograph system to perform pressure myograph testing on the murine vagina and cervix. Utilizing media with and without calcium, the contributions of the smooth muscle cells (SMC) basal tone and passive extracellular matrix (ECM) were isolated for the organs under estimated physiological conditions.

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