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Chapitres
- 00:04Titre
- 01:16crRNA Design and Preparation of Cas9/gRNA RNP Complexes
- 02:16Electroporation of RNP Complexes into OCI-AML3 Cells
- 03:12Screening and Selection of Cell Clones with Biallelic Deletions
- 05:51Functional Analyses of Silencer Deletions with Real-time Quantitative RT-PCR
- 07:18Results: RUNX1 Intronic Silencer Deletion
- 08:16Conclusion
Direkte levering av forhåndsmonterte Cas9/guide RNA ribonucleoprotein komplekser er en rask og effektiv måte for Genova redigering i blodkreft celler. Her bruker vi denne tilnærmingen til å slette en RUNX1 intronic lyddemper og undersøke transcriptional svar i OCI-AML3 Leukemic celler.
