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Quantification of Coenzyme A in Cells and Tissues
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Journal JoVE Biochimie
Quantification of Coenzyme A in Cells and Tissues

Quantification of Coenzyme A in Cells and Tissues

DOI:
10.3791/60182-v

•

08:51 min

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September 27, 2019

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, , ,
1Department of Infectious Diseases,St. Jude Children’s Research Hospital

Chapitres

  • 00:05Titre
  • 01:30Extraction and Derivatization of CoA (Coenzyme A) in Cultured Cells
  • 03:32Extraction & Derivatization of CoA in Tissues
  • 05:31Sample Clean-up with Solid Phase Extraction Column
  • 06:47Results: HPLC Profiles and CoA-bimane Standard Curves
  • 07:45Conclusion

Summary

Traduction automatique

Traduction automatique

This method describes sample preparation from cultured cells and animal tissues, extraction and derivatization of coenzyme A in the samples, followed by high pressure liquid chromatography for purification and quantification of the derivatized coenzyme A by absorbance or fluorescence detection.

Tags

Coenzyme ACoACellular MetabolismNeurodegenerationBrain Iron AccumulationCoA Biosynthetic PathwaySample PreparationSolid-phase ExtractionCell CulturePotassium HydroxideTrizma-hydrochlorideMBBrThiol

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