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1Marine Biological Section, Department of Biology,University of Copenhagen, 2Center for Electromicrobiology,Aarhus University, 3PCO AG, 4Climate Change Cluster,University of Technology Sydney, 5Aarhus University Centre for Water Technology, Section for Microbiology, Department of Bioscience,Aarhus University
Chapitres
- 00:05Titre
- 00:51Planar Oxygen (O2) Optode Fabrication
- 01:47Rhizo-Sandwich Chamber Preparation
- 04:01Imaging Setup
- 04:45Camera Settings and Operation
- 05:40O2 Optode Calibration and Sample Imaging
- 06:41Results: Representative Optode Calibration and Lifetime O2 Distribution Imaging
- 07:46Conclusion
Descrevemos o uso de uma nova câmera vitalícia de luminescência de domínio de frequência para mapear distribuições 2D O2 com folhas de sensor óptico. Os procedimentos de análise do sistema de câmera e de imagem são descritos juntamente com a preparação, calibração e aplicação de folhas de sensor para visualizar o microambiente O2 na rizosfera das plantas aquáticas.
Tags
Luminescence Lifetime ImagingOxygenFrequency-domain-based Camera SystemOptodePlanar Oxygen Sensitive OptodeRhizo-sandwich ChamberOxygen DistributionSpatial And Temporal ResolutionElectron AcceptorBiological SystemsEcologyMedical ResearchBioprintingPressure-sensitive PrintingCocktail OptimizationPET FoilMicroscope SlidesAcrylic-based Instant AdhesiveAir Bubbles
