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Nanoscopic Imaging of Human Tissue Sections via Physical and Isotropic Expansion
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Médecine
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Journal JoVE Médecine
Nanoscopic Imaging of Human Tissue Sections via Physical and Isotropic Expansion

Nanoscopic Imaging of Human Tissue Sections via Physical and Isotropic Expansion

DOI:
10.3791/60195-v

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09:11 min

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September 25, 2019

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, , ,
1Department of Biological Sciences,Carnegie Mellon University, 2QPathology

Chapitres

  • 00:05Titre
  • 01:19Preparation of Archived and Freshly Prepared Clinical Tissue Slides for ExPath
  • 04:05In Situ Polymerization of Specimens
  • 05:16Sample Digestion and Imaging
  • 06:40Results: Multicolor Super-resolution Tissue Imaging using Expansion Pathology
  • 08:18Conclusion

Summary

Traduction automatique

Traduction automatique

Nanoscale imaging of clinical tissue samples can improve understanding of disease pathogenesis. Expansion pathology (ExPath) is a version of expansion microscopy (ExM), modified for compatibility with standard clinical tissue samples, to explore the nanoscale configuration of biomolecules using conventional diffraction limited microscopes.

Tags

Keywords: Nanoscopic ImagingExpansion PathologyExPathFluorescent LabelingBiomoleculesDisease-relatedCancerBrain DiseaseAutoimmune DiseaseFFPEAntigen RetrievalHydrogelPhysical ExpansionIsotropic ExpansionDiffraction LimitConventional MicroscopySample Preparation

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