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Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip
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Bio-ingénierie
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Journal JoVE Bio-ingénierie
Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip

DOI:
10.3791/60202-v

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07:05 min

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September 27, 2019

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, , ,
1Institute of Biomedical Engineering and Nanomedicine,National Health Research Institutes, 2Ph.D. Program in Tissue Engineering and Regenerative Medicine,National Chung Hsing University, 3National Institute of Cancer Research,National Health Research Institutes

Chapitres

  • 00:04Titre
  • 00:35PDMS Device Preparation for Multiple Single Cell Capture
  • 02:23Preparation of a Single-cell Suspension
  • 03:39Multiple Single-cell Capture & Triple Single-cell Capture
  • 04:48Results: Single Cell Capture and Cell Co-culture in the Hydrodynamic Shuttling Chip
  • 06:08Conclusion

Summary

Traduction automatique

Traduction automatique

This report describes a microfluidic chip-based method to set up a single cell culture experiment in which high-efficiency pairing and microscopic analysis of multiple single cells can be achieved.

Tags

Single-cell Co-cultureMicrofluidic ChipPDMS Device FabricationCell CaptureCell-cell InteractionLive Cell ImagingDeterministic Cell Patterning

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