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Valutazione della capacità di differenziazione delle cellule epiteliali della prostata del topo utilizzando la cultura organoide
This content is Free Access.
1Molecular Biology Interdepartmental Program,University of California, Los Angeles, 2Department of Molecular, Cell, and Developmental Biology,University of California, Los Angeles, 3Department of Urology, David Geffen School of Medicine,University of California, Los Angeles, 4Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research,University of California, Los Angeles, 5Jonsson Comprehensive Cancer Center,University of California, Los Angeles, 6Molecular Biology Institute,University of California, Los Angeles
Chapitres
- 00:05Titre
- 00:52Plating Sorted Prostate Epithelial Cells into Primary Mouse Organoid Culture
- 03:29Extracting Protein Lysate from Prostate Organoids for Western Blot Analysis
- 04:50Fixing and Staining Prostate Organoids for Immunohistochemical Analysis by Whole-Mount Confocal Microscopy
- 06:44Tissue Clearing and Mounting of the Stained Prostate Organoids for Whole-mount Confocal Microscopy
- 07:57Results: Analysis of Lineage Marker Expression in Prostate Organoids by Western Blot and Whole-Mount Confocal Microscopy
- 09:44Conclusion
Gli organoid della prostata del topo rappresentano un contesto promettente per valutare i meccanismi che regolano la differenziazione. Questo documento descrive un approccio migliorato per stabilire gli organoidi della prostata e introduce metodi per (1) raccogliere il lisato proteico dagli organoidi e (2) fissare e macchiare gli organoidi per la microscopia confocale a cavalcatura completa.
