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Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes
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Neurosciences
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Journal JoVE Neurosciences
Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes

Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes

DOI:
10.3791/60230-v

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00:07 min

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August 28, 2019

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, ,
1Department of Cell Biology and Physiology,University of North Carolina at Chapel Hill

Chapitres

  • 00:04Titre
  • 00:31Fluorescently-Tagged Plasmid Transfection and Fluorescent Probe Late Endosome/Lysosome Labeling
  • 02:13Time-Lapse Imaging
  • 03:03Image Analysis
  • 05:00Results: Representative Quantitative Analyses of Cargo Dynamics in Astrocytes
  • 06:38Conclusion

Summary

Traduction automatique

Traduction automatique

Here we describe an in vitro live-imaging method to visualize intracellular transport of organelles and trafficking of plasma membrane proteins in murine astrocytes. This protocol also presents an image analysis methodology to determine cargo transport itineraries and kinetics.

Tags

Keywords: Intracellular TransportOrganelle MotilityAstrocytesLive ImagingFluorescent LabelingLipofectionTransfectionTime-lapse MicroscopyImage Analysis

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