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Cell type-spesifikke Gene Expression profilering i musen Liver
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Journal JoVE Biologie
Cell Type-specific Gene Expression Profiling in the Mouse Liver

Cell type-spesifikke Gene Expression profilering i musen Liver

DOI:
10.3791/60242-v

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10:06 min

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September 17, 2019

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, ,
1Department of Genetics,University of Pennsylvania, 2Department of Medicine,University of Pennsylvania

Chapitres

  • 00:04Titre
  • 01:21Conjugation of Antibodies to Magnetic Beads
  • 03:36Liver Tissue Lysis
  • 05:33Immunoprecipitation and RNA Isolation
  • 07:37Results: Cell Type-specific Isolation of High-quality RNA by Trap
  • 09:03Conclusion

Summary

Traduction automatique

Traduction automatique

Oversette ribosom affinitet rensing (TRAP) muliggjør rask og effektiv isolering av celle type-spesifikke oversette mRNA. Her viser vi en metode som kombinerer hydrodynamisk hale-blodåre injeksjon i en mus modell av leveren Repopulation og TRAP å undersøke uttrykket profilen til repopulating hepatocytter.

Tags

Keywords: TRAP-seqCell Type-specificGene ExpressionLiverHepatocytesLiver InjuryCell TypesMagnetic BeadsProtein LAntibody

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