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Multi-Fiber Photometry to Record Neural Activity in Freely-Moving Animals
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Neurosciences
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Journal JoVE Neurosciences
Multi-Fiber Photometry to Record Neural Activity in Freely-Moving Animals

Multi-Fiber Photometry to Record Neural Activity in Freely-Moving Animals

DOI:
10.3791/60278-v

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05:52 min

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October 20, 2019

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, ,
1CERVO Brain Research Center, Department of Psychiatry and Neurosciences,Université Laval, 2Center for Neural Circuits and Behavior, Department of Neuroscience and Section of Neurobiology, Division of Biology,University of California at San Diego, 3Neurophotometrics Ltd.

Chapitres

  • 00:04Titre
  • 00:49Optical Path Between CMOS and Patch Cord
  • 01:58Setup of ROIs and Recording Arena
  • 03:09In-vivo and Dual-color Recordings
  • 04:52Results: Florescence Increased After Administration of Air Puffs
  • 05:18Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol details how to implement and perform multi-fiber photometry recordings, how to correct for calcium-independent artifacts, and important considerations for dual-color photometry imaging.

Tags

Keywords: Multi-fiber PhotometryFiber PhotometryCSOM ImagingNeural ActivityFreely-moving AnimalsGenetically Encoded Custom IndicatorOptical FibersFive-axis TranslatorCMOS CameraRegions Of Interest (ROIs)Fiber LabelingRecording Arena Setup

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