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Immunologie et infection
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Visualization of Candida albicans in the Murine Gastrointestinal Tract Using Fluorescent In Situ Hybridization
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Visualization of Candida albicans in the Murine Gastrointestinal Tract Using Fluorescent In Situ Hybridization

Visualization of Candida albicans in the Murine Gastrointestinal Tract Using Fluorescent In Situ Hybridization

DOI:
10.3791/60283-v

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10:08 min

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November 05, 2019

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, ,
1Department of Microbiology and Immunology,University of California, San Francisco, 2Department of Medicine, Division of Infectious Disease,University of California, San Francisco

Chapitres

  • 00:04Titre
  • 01:28Gastrointestinal Colonization of Mice with C. albicans Using Oral Gavage
  • 03:30Preparation of GI Tissues for Histology
  • 05:50FISH Staining in GI Tract Tissues
  • 08:22Results: FISH-stained C. albicans In Vitro and in Different Gut Compartments
  • 09:17Conclusion

Summary

Traduction automatique

Traduction automatique

The purpose of this protocol is to visualize Candida albicans cell shape and localization in the mammalian gastrointestinal tract.

Tags

Candida AlbicansFluorescent In Situ HybridizationGastrointestinal TractMurineVisualizationMicrobiotaInflammatory Bowel DiseaseGavageDissectionAnimal Model

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