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JoVE Journal Cancer Research
Fibroblast-afledt 3D Matrix system gældende for Endotelrørdannelse analyse
 
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Fibroblast-afledt 3D Matrix system gældende for Endotelrørdannelse analyse

Article DOI: 10.3791/60304-v • 07:21 min • December 26th, 2019
December 26th, 2019 •
1, 2, 3, 1, 4, 1
1Medical Oncology Department, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), CIBERONC, 2Department of Medical Oncology, Hospital Universitario Puerta de Hierro de Majadahonda, 3Instituto de Investigaciones Biomédicas Alberto Sols, Consejo Superior de Investigaciones Científicas - Universidad Autónoma de Madrid, CIBERONC, 4Department of Oncology & Pathology, Karolinska Institutet

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Formålet med denne metode er at opnå fibroblast-afledte 3D matricer som et naturligt stillads for efterfølgende cellulære assays. Fibroblaster siver i en forbehandlet kultur plade og stimuleres med ascorbinsyre til matrix generering. Matricer er decellularized og blokeret for kultur relevante celler (f. eks endotelceller).

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Kræftforskning tumor mikromiljø tredimensionel (3D) kultur ekstracellulær matrix fibroblaster kollagen Neoangiogenesis
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