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섬유 아세포 유래 3D 매트릭스 시스템은 내피 튜브 형성 분석에 적용
Journal JoVE
Recherche en cancérologie
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Journal JoVE Recherche en cancérologie
Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay
DOI:

07:21 min

December 26, 2019

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Chapitres

  • 00:04Titre
  • 01:13Preparation of Solutions and Cell Cultures
  • 02:32Fibroblast-derived 3D Matrices
  • 04:50Tube Formation Assay
  • 05:45Results: Effects of PDGF Treatment on ECM Formation and Endothelial Cell Activation
  • 06:42Conclusion

Summary

Traduction automatique

The aim of this method is to obtain fibroblast-derived 3D matrices as a natural scaffold for subsequent cellular assays. 섬유아세포는 전처리된 배양판에 씨를 뿌리고 매트릭스 생성을 위한 아스코르브산으로 자극된다. 행렬은 탈세포화되고 관련 세포(예를 들어, 내피 세포)를 배양하도록 차단된다.

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