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Simultaneous Flow Cytometric Characterization of Multiple Cell Types Retrieved from Mouse Brain/Spinal Cord Through Different Homogenization Methods
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Médecine
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Journal JoVE Médecine
Simultaneous Flow Cytometric Characterization of Multiple Cell Types Retrieved from Mouse Brain/Spinal Cord Through Different Homogenization Methods

Simultaneous Flow Cytometric Characterization of Multiple Cell Types Retrieved from Mouse Brain/Spinal Cord Through Different Homogenization Methods

DOI:
10.3791/60335-v

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10:24 min

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November 19, 2019

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, , ,
1Dana-Farber/Boston Children’s Cancer and Blood Disorders Center, 2Harvard Medical School

Chapitres

  • 00:04Titre
  • 00:49Enzymatic Brain and Spinal Cord Digestion
  • 03:58Mechanical Brain and Spinal Cord Homogenization
  • 05:00Debris Removal
  • 06:19Flow Cytometric Cell Staining
  • 07:48Results: Representative Relative Proportions of Different Cell Types Retrieved from the CNS According to the Tissue Homogenization Method
  • 09:38Conclusion

Summary

Traduction automatique

Traduction automatique

We present a flow cytometry method to identify simultaneously different cell types retrieved from mouse brain or spinal cord. This method could be exploited to isolate or characterize pure cell populations in neurodegenerative diseases or to quantify the extent of cell targeting upon in vivo administration of viral vectors or nanoparticles.

Tags

Keywords: Flow CytometryCell CharacterizationMouse BrainSpinal CordCell IsolationGene DeliveryDrug DeliveryNeurodegenerative Disorders

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