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Chapitres
- 00:04Titre
- 01:12Large Scale Expression of Histidine-6 (His6)-Tagged Nuclear Factor-kappa-B Essential Modulator Crystallography Construct (NEMO-EEAA)
- 02:33His6-Tagged NEMO-EEAA Purification
- 04:42His6 Tag Cleavage and Purification
- 07:09Sparse Matrix Screening
- 08:23Seed Stock Generation
- 09:00Crystal Looping
- 09:43X-Ray Data Processing and Structure Solution
- 10:38Results: Representative Production, Crystallization, and Structure Determination of the IKK-Binding Domain of NEMO
- 12:28Conclusion
Vi beskriver protokoller for strukturen bestemmelse af IKK-bindende domæne NEMO af X-ray crystallography. Metoderne omfatter protein ekspression, rensning og karakterisering samt strategier for vellykket krystal optimering og struktur bestemmelse af proteinet i sin ubundne form.
