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High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
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Biochimie
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Journal JoVE Biochimie
High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

DOI:
10.3791/60378-v

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14:09 min

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November 16, 2019

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, , , ,
1Department of Physics and Technology,UiT The Arctic University of Norway, 2Vascular Biology Research Group, Department of Medical Biology,UiT The Arctic University of Norway

Chapitres

  • 00:01Titre
  • 01:13Waveguide Cleaning
  • 02:05Sample Chamber Preparation
  • 02:41Fluorescent Labelling
  • 04:12Setup Components
  • 04:56Waveguide Coupling
  • 07:26Diffraction Limited Imaging
  • 08:42dSTORM Imaging
  • 09:35dSTORM Image Reconstruction
  • 10:42Résultats
  • 12:27Conclusion

Summary

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Traduction automatique

Chip-based super-resolution optical microscopy is a novel approach to fluorescence microscopy and offers advantages in cost effectiveness and throughput. Here, the protocols for chip preparation and imaging are shown for TIRF microscopy and localization-based super-resolution microscopy.

Tags

Keywords: Photonic ChipTIRF MicroscopyDSTORMEvanescent WaveHigh-throughput ImagingWaveguidePDMSSample PreparationCell Seeding

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