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A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells
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Biologie du développement
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Journal JoVE Biologie du développement
A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells

A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells

DOI:
10.3791/60379-v

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08:30 min

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March 04, 2020

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, , , , ,
1Department of Pathology,Yale University School of Medicine, 2Stem Cell Center,Yale University School of Medicine

Chapitres

  • 00:05Introduction
  • 00:53Stem Cell Maintenance
  • 03:03Dissociation of the hESCs for Organoid Culture
  • 04:27Generation of Organoids
  • 06:06Results: Overview of Organoid Growth Conditions and Morphology
  • 07:37Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol was generated as a means to produce brain organoids in a simplified, low cost manner without exogenous growth factors or basement membrane matrix while still maintaining the diversity of brain cell types and many features of cellular organization.

Tags

Keywords: Brain OrganoidsHuman Embryonic Stem CellsNeurodevelopmental DisordersToxic InsultsBrain CancerReproducibleSimple WorkflowCost-effectiveMatrixH9 HESCsMTeSR1 MediaLow Oxygen IncubatorProtease SolutionPassaging

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