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Lab-on-a-CD-platform til generering af flercellede tredimensionelle Sfæroider
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Journal JoVE Bio-ingénierie
Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids

Lab-on-a-CD-platform til generering af flercellede tredimensionelle Sfæroider

DOI:
10.3791/60399-v

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10:27 min

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November 07, 2019

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, , , ,
1Department of Mechanical Engineering, Graduate School,Chung-Ang University, 2Department of Biomedical Engineering, College of Medicine,Seoul National University

Chapitres

  • 00:05Titre
  • 00:28Centrifugal Microfluidic-Based Spheroid (CMS) Culture Chip Fabrication
  • 02:06Cell Preparation
  • 03:10Cell Staining
  • 03:45Monoculture Spheroid Formation
  • 05:33Co-Culture Spheroid Formation: Concentric Spheroid Formation
  • 06:28Co-Culture Spheroid Formation: Janus Spheroid Formation
  • 07:33Co-Culture Spheroid Formation: Sandwich Spheroid Formation
  • 08:48Results: Representative CMS Culture Chip Spheroid Culture Analyses
  • 09:51Conclusion

Summary

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Traduction automatique

Vi præsenterer en motordrevet centrifugal mikrofluidic enhed, der kan dyrke celle sfæroider. Ved hjælp af denne enhed, sfæroider af enkelt eller flere celletyper kunne let cocultureres under høj tyngdekraft betingelser.

Tags

Lab-on-a-CD PlatformMulticellular Three-dimensional SpheroidsCentrifugal ForceMicrowellsPDMSPlasma CleaningCell CultureCell DetachmentCell Labeling

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