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Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations
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Biochimie
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Journal JoVE Biochimie
Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations

Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations

DOI:
10.3791/60402-v

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10:26 min

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November 07, 2019

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, , , , , , ,
1Aix-Marseille Univ, INSERM, CNRS,Institut Paoli-Calmettes, CRCM

Chapitres

  • 00:04Titre
  • 01:43Generation of Stable Cell Lines Expressing 6His-Flag-Ubl
  • 03:36Double Purification of Modified Proteins
  • 06:25Processing of Mass Spectrometry Data to Generate Profiles of Ub/Ubls PTMs (Ubiquitin/Ubiquitin-likes Post-Translational Modifications)
  • 07:45Results: Ubiquitinated Proteins
  • 09:13Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol aims at establishing ubiquitin (Ub) and ubiquitin-likes (Ubls) specific proteomes in order to identify alterations of these kind of post-translational modifications (PTMs), associated with a specific condition such as a treatment or a phenotype.

Tags

Keywords: UbiquitinUbiquitin-likePost-translational ModificationsProfilingLentivirusHEK 293T CellsTransfectionProtein PurificationEukaryotic ModelsDiagnosisPrognosisMolecular Targets

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