Journal
/
Génétique
/
Mapping Alzheimer’s Disease Variants to Their Target Genes Using Computational Analysis of Chromatin Configuration
Journal JoVE
Génétique
This content is Free Access.
Journal JoVE Génétique
Mapping Alzheimer’s Disease Variants to Their Target Genes Using Computational Analysis of Chromatin Configuration

Mapping Alzheimer’s Disease Variants to Their Target Genes Using Computational Analysis of Chromatin Configuration

DOI:
10.3791/60428-v

•

04:41 min

•

January 09, 2020

•

, , ,
1Department of Genetics,University of North Carolina, 2Neuroscience Center,University of North Carolina, 3Thurston Arthritis Research Center,University of North Carolina, 4Department of Cell Biology and Physiology,University of North Carolina

Chapitres

  • 00:05Titre
  • 01:00Generation of a Granges Object for Credible SNPs and Positional Mapping
  • 01:53Developmental Expression Trajectories
  • 02:18Cell-type Expression Profiles and Gene Annotation Enrichment Analysis
  • 02:46Results: Determination and Characterization of Putative Target Genes of AD GWS Loci
  • 03:52Conclusion

Summary

Traduction automatique

Traduction automatique

We present a protocol to identify functional implications of non-coding variants identified by genome-wide association studies (GWAS) using three-dimensional chromatin interactions.

Tags

Keywords: Alzheimer’s DiseaseGWASChromatin ConfigurationComputational AnalysisTarget GenesSNPsHi-CGene ExpressionCell-type ExpressionGene Annotation Enrichment

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/60428?article_type=v

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
check_url/fr/60428?article_type=v

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes
check_url/fr/60428?article_type=v

Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation
check_url/fr/60428?article_type=v

Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions
check_url/fr/60428?article_type=v

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
check_url/fr/60428?article_type=v

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
check_url/fr/60428?article_type=v

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
check_url/fr/60428?article_type=v

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
check_url/fr/60428?article_type=v

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila
check_url/fr/60428?article_type=v

Identification of Circular RNAs using RNA Sequencing

Read Article