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Pancreatic Tissue-Derived Extracellular Matrix Bioink for Printing 3D Cell-Laden Pancreatic Tissue Constructs
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1Department of Mechanical Engineering,Pohang University of Science and Technology, 2School of Interdisciplinary Bioscience and Bioengineering,Pohang University of Science and Technology, 3Asan Institute for Life Science,University of Ulsan College of Medicine and Asan Medical Center, 4Division of Hepato-Biliary and Pancreatic Surgery, Department of Surgery,University of Ulsan College of Medicine and Asan Medical Center, 5Department of Creative IT Engineering,Pohang University of Science and Technology
Chapitres
- 00:04Titre
- 01:10Tissue Decellularization
- 02:50Pancreatic Tissue Derived Decellularized Extracellular Matrix (pdECM) Bioink Preparation
- 03:453D Pancreatic Construct Cell Printing with Patterned Structure
- 05:11Results: Representative Biochemical Characterization and Rheological Analysis of pdECM Bioink
- 06:50Conclusion
Decellularized extracellular matrix (dECM) can provide suitable microenvironmental cues to recapitulate the inherent functions of target tissues in an engineered construct. This article elucidates the protocols for the decellularization of pancreatic tissue, evaluation of pancreatic tissue-derived dECM bioink, and generation of 3D pancreatic tissue constructs using a bioprinting technique.
