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Brug af Tg(Vtg1
 
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Brug af Tg(Vtg1:mcherry) Zebrafisk embryoner til at teste de østrogene virkninger af hormonforstyrrende forbindelser

Article DOI: 10.3791/60462-v • 12:02 min • August 8th, 2020
August 8th, 2020 •
1, 1, 1, 1, 1, 1, 1, 1, 2, 1
1Department of Aquaculture, Institute of Aquaculture and Environmental Safety, Faculty of Agricultural and Environmental Sciences, Szent István University, 2Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham

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Til stede her er en detaljeret protokol for brugen af zebrafisk embryoner Tg (vtg1: mCherry) til påvisning af østrogene effekter. Protokollen dækker formering af fisk og behandling af embryoner, og understreger detektion, dokumentation, og evaluering af fluorescerende signaler induceret af hormonforstyrrende stoffer (EDC).

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Miljøvidenskab hormonforstyrrende stoffer EDC zearalenon zearalenol biomonitor bioindicator xenoestrogens vitellogenin
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