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Recherche en cancérologie
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Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids
Journal JoVE
Recherche en cancérologie
This content is Free Access.
Journal JoVE Recherche en cancérologie
Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids

Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids

DOI:
10.3791/60469-v

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08:43 min

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January 31, 2020

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, , ,
1Department of Life Sciences,Pohang University of Science and Technology

Chapitres

  • 00:04Titre
  • 00:50In Vitro Culture of Bladder Tumor Organoids
  • 03:50Genetic Manipulation of Bladder Organoids via Lentivirus-mediated Transduction
  • 04:36Orthotopic Transplantation of Bladder Organoids
  • 07:06Results: Culture and Transplantation of Bladder Tumor Organoids
  • 08:01Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol provides detailed experimental steps to establish a three-dimensional in vitro culture of bladder tumor organoids derived from carcinogen-induced murine bladder cancer. Culture methods including passaging, genetic engineering, and orthotopic transplantation of tumor organoids are described.

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Keywords: Tumor OrganoidsBladder CancerCell CultureGenetic ManipulationOrthotopic TransplantationTumor Microenvironment

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