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小鼠膀胱肿瘤器官的培养、操作和正交移植
Journal JoVE
Recherche en cancérologie
This content is Free Access.
Journal JoVE Recherche en cancérologie
Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids
DOI:

08:43 min

January 31, 2020

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Chapitres

  • 00:04Titre
  • 00:50In Vitro Culture of Bladder Tumor Organoids
  • 03:50Genetic Manipulation of Bladder Organoids via Lentivirus-mediated Transduction
  • 04:36Orthotopic Transplantation of Bladder Organoids
  • 07:06Results: Culture and Transplantation of Bladder Tumor Organoids
  • 08:01Conclusion

Summary

Traduction automatique

该协议提供了详细的实验步骤,以建立从致癌性鼠膀胱癌衍生的膀胱肿瘤器官的三维体外培养。介绍了肿瘤器官的传体内、遗传工程、正交移植等培养方法。

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