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Isolation og kultur af menneskelige modne adipocytter Ved hjælp af membran modne Adipocyte aggregatkulturer (MAAC)
Journal JoVE
Biologie
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Journal JoVE Biologie
Isolation and Culture of Human Mature Adipocytes Using Membrane Mature Adipocyte Aggregate Cultures (MAAC)

Isolation og kultur af menneskelige modne adipocytter Ved hjælp af membran modne Adipocyte aggregatkulturer (MAAC)

DOI:
10.3791/60485-v

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06:28 min

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February 13, 2020

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, ,
1Bioscience Metabolism, Research and Early Development, Cardiovascular, Renal and Metabolism (CVRM), BioPharmaceuticals R&D,AstraZeneca, 2The Lundberg Laboratory for Diabetes Research,University of Gothenburg, 3Wallenberg Centre for Molecular and Translational Medicine,University of Gothenburg

Chapitres

  • 00:05Introduction
  • 00:53Human Subcutaneous Adipose Tissue Dissection
  • 01:32Collagenase Treatment
  • 02:21Mature Adipocyte Purification
  • 03:20Mature Adipocyte Packing
  • 03:51Mature Adipocyte Seeding and Maintenance
  • 04:50Results: Representative Mature Adipocyte Aggregate Culture (MAAC) Appearance and Response to Diverse Pharmacological Agonism
  • 05:55Conclusion

Summary

Traduction automatique

Traduction automatique

Membran modne adipocyte samlede kultur (MAAC) er en ny metode til kultur modne menneskelige adipocytter. Her beskriver vi, hvordan du isolerer adipocytter fra menneskelig fedt, og hvordan du konfigurerer MAAC.

Tags

Mature AdipocyteAdipocyte CultureMembrane Mature Adipocyte Aggregate Cultures (MAAC)Adipose BiologyDiabetesObesityCell InteractionsAdipocyte IsolationAdipocyte SuspensionAdipocyte WashAdipocyte Separation

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