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Developmental Biology

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JoVE Journal Developmental Biology
Laser opsamling Microdissection af mus embryonale brusk og knogle for genekspression analyse
 
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Laser opsamling Microdissection af mus embryonale brusk og knogle for genekspression analyse

Article DOI: 10.3791/60503-v • 09:20 min • December 18th, 2019
December 18th, 2019 •
1, 1, 1,2, 1, 1
1Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, 2Icahn Institute for Data Science and Genomic Technology, Icahn School of Medicine at Mount Sinai

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Denne protokol beskriver laser opsamling microdissection til isolering af brusk og knogler fra friske frosne sektioner af muse embryonet. Brusk og knogler kan hurtigt visualiseres ved cresylacetat violet farvning og indsamles netop for at give høj kvalitet RNA for transcriptomic analyse.

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Udviklingsmæssige biologi laser opsamling microdissection Meckel brusk mandibulær knogle cresylacetat violet RNA isolation RNA sekventering
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