Journal
/
Biologie du développement
/
In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
Journal JoVE
Biologie du développement
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie du développement
In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

DOI:
10.3791/60551-v

•

10:00 min

•

November 28, 2019

•

, , , , , ,
1Departamento de Fisiología y Desarrollo Celular,Instituto Nacional de Perinatología, 2Biotecnología Médica y Farmacéutica CONACYT,Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), 3Departamento de Inmunobioquímica,Instituto Nacional de Perinatología, 4Instituto de Neurobiología, UNAM

Chapitres

  • 00:04Titre
  • 00:43Mechanical Amniotic Membrane Separation
  • 02:40Membrane Washing
  • 03:36Enzymatic Amniotic Membrane Digestion
  • 06:21Human Amniotic Epithelial Cells (HAEC) Isolation and Culture
  • 07:31Results: Representative HEAC Morphologic and Phenotypic Analyses
  • 09:12Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol describes the isolation of epithelial cells from different anatomical regions of the human amniotic membrane to determine their heterogeneity and functional properties for possible application in clinical and physiopathological models.

Tags

Keywords: In Vitro CultureEpithelial CellsHuman Amniotic MembraneAnatomical RegionsUmbilical AmnionPlacental AmnionReflected RegionDissectionSaline WashCell Isolation

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/60551?article_type=v

Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos
check_url/fr/60551?article_type=v

Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition
check_url/fr/60551?article_type=v

Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol
check_url/fr/60551?article_type=v

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
check_url/fr/60551?article_type=v

Isolation of Mouse Endometrial Epithelial and Stromal Cells for In Vitro Decidualization
check_url/fr/60551?article_type=v

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
check_url/fr/60551?article_type=v

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
check_url/fr/60551?article_type=v

Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells
check_url/fr/60551?article_type=v

Application of Aorta-gonad-mesonephros Explant Culture System in Developmental Hematopoiesis
check_url/fr/60551?article_type=v

Culturing of Retinal Pigment Epithelial Cells on an Ex Vivo Model of Aged Human Bruch’s Membrane

Read Article