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Assessment of the Acute Inhalation Toxicity of Airborne Particles by Exposing Cultivated Human Lung Cells at the Air-Liquid Interface
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Médecine
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Journal JoVE Médecine
Assessment of the Acute Inhalation Toxicity of Airborne Particles by Exposing Cultivated Human Lung Cells at the Air-Liquid Interface

Assessment of the Acute Inhalation Toxicity of Airborne Particles by Exposing Cultivated Human Lung Cells at the Air-Liquid Interface

DOI:
10.3791/60572-v

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10:10 min

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February 23, 2020

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, , , , , , , , , ,
1Bundeswehr Institute of Pharmacology and Toxicology, 2Walther Straub Institute of Pharmacology and Toxicology,University of Munich, 3Cultex Laboratories GmbH, 4Cultex Technologies GmbH (formerly Cultex Laboratories GmbH), 5seh consulting + services

Chapitres

  • 00:05Introduction
  • 00:31Seeding of Cells onto Microporous Membranes in Cell Culture Inserts
  • 01:58Pressing of Test Substances
  • 03:07Leakage Test of the Radial Flow System
  • 03:51Aerosol Generation
  • 05:09Exposure Experiments
  • 07:30Cell Viability
  • 08:27Results: Viability Data
  • 09:11Conclusion

Summary

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Traduction automatique

We present a robust, transferable and predictive in vitro exposure system for the screening and monitoring of airborne particles concerning their acute pulmonary cytotoxicity by exposing cultivated human lung cells at the air-liquid interface (ALI).

Tags

Keywords: Acute Inhalation ToxicityAirborne ParticlesCultivated Human Lung CellsAir-liquid InterfaceIn Vitro Exposure SystemRadial Aerosol DistributionA549 CellsCell Culture InsertsCompressed Air PressureParticulate Exposures

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