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In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction
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Immunologie et infection
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Journal JoVE Immunologie et infection
In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction

In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction

DOI:
10.3791/60585-v

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08:02 min

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January 22, 2020

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, , , ,
1Division of Regenerative Medicine, Department of Medicine,Loma Linda University, 2Zhengzhou University, 3Department of Veterinary Biomedical Sciences, College of Veterinary Medicine,Long Island University, 4Jinan Infectious Disease Hospital,Shandong University

Chapitres

  • 00:05Titre
  • 00:571-alpha-Hydroxylase and Retinaldehyde Dehydrogenase 2 (RALDH2) Lentivirus (Lenti-CYP-ALDH virus) Preparation
  • 03:04Bone Marrow-Derived Dendritic Cell (BMDC) Generation
  • 05:06BMDC Lenti-CYP-ALDH Transduction
  • 06:00Results: Representative Gut-Homing Regulatory T Cell Induction
  • 07:38Conclusion

Summary

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Traduction automatique

Here we present a protocol for in vivo augmentation of gut-homing regulatory T cell induction. In this protocol, dendritic cells are engineered to locally produce high concentrations of the active vitamin D (1,25-dihydroxyvitamin D or 1,25[OH]2D) and the active vitamin A (retinoic acid or RA) de novo.

Tags

Gut-homing Regulatory T CellsIn Vivo AugmentationDendritic Cell GenerationLentiviral TransductionVitamin DVitamin APeripheral Immune System

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