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Generering af kontrollerede, dynamiske kemiske landskaber til undersøgelse af mikrobiel adfærd
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1Institute of Environmental Engineering, Department of Civil, Environmental and Geomatic Engineering, 2School of Mathematics and Statistics,University of Melbourne, 3Institute of Marine Sciences,University of California, Santa Cruz, 4Faculty of Life and Environmental Sciences,University of Tsukuba, 5Microbiology Research Center for Sustainability,University of Tsukuba, 6Department of Ecology and Evolutionary Biology,Princeton University
Chapitres
- 00:05Introduction
- 00:35Fabrication of the Microfluidic Device for the Single Chemical-pulse Experiment
- 02:02Fabrication of the 3D-printed Millifluidic Device for the Experiment with Multiple Pulses
- 05:28Multiple Chemical-pulse Experiment
- 07:11Results: Bacterial Responses to a Chemical Pulse and Swimming Statistics in the Absence of Chemical Gradients
- 08:50Conclusion
En protokol for produktion af dynamiske kemiske landskaber ved fotolyse inden for mikrofluidic og millifluidic opsætninger præsenteres. Denne metode er velegnet til at studere forskellige biologiske processer, herunder motiladfærd, optagelse af næringsstoffer eller tilpasning til kemikalier af mikroorganismer, både på enkeltcelle- og befolkningsniveau.
