Journal
/
Biochimie
/
Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Journal JoVE
Biochimie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biochimie
Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

DOI:
10.3791/60616-v

•

08:56 min

•

March 08, 2020

•

, , ,
1Department of Biology,Concordia University, 2Department of Chemistry and Biochemistry, Centre for Biological Applications of Mass Spectrometry,Concordia University

Chapitres

  • 00:05Introduction
  • 00:34Yeast Culture and Lipid Extraction
  • 03:55Extracted Lipid Separation
  • 05:26Mass Spectrometric Analysis and Lipid Class Identification and Quantification
  • 06:53Results: Representative Quantitative Analysis of Saccharomyces Cerevisiae Cellular Lipidomes
  • 08:19Conclusion

Summary

Traduction automatique

Traduction automatique

We present a protocol using liquid chromatography coupled with tandem mass spectrometry to identify and quantify major cellular lipids in Saccharomyces cerevisiae. The described method for a quantitative assessment of major lipid classes within a yeast cell is versatile, robust, and sensitive.

Tags

LipidomeSaccharomyces CerevisiaeLiquid ChromatographyTandem Mass SpectrometryLipid ExtractionYeast CultureInternal Lipid StandardsQuantitative Analysis

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics

Read Article