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Biochemistry

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JoVE Journal Biochemistry
Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors
 

Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors

Article DOI: 10.3791/60619-v • 09:11 min • November 23rd, 2019
November 23rd, 2019 •
*1, *2, 2, 2, 2, 2, 2, 1,3
1Research Program in Molecular and Integrative Biosciences, University of Helsinki, 2Drug Research Program, Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, 3School of Biomedical Sciences and Astbury Centre for Structural Molecular Biology, University of Leeds
* These authors contributed equally

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résumé
Automatic Translation

Here we present a screening method for membrane-bound pyrophosphatase (from Thermotoga maritima) inhibitors based on the molybdenum blue reaction in a 96 well plate format.

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Thermotoga Maritima Membrane-bound Pyrophosphatase Inhibitors Compounds Parasitic Diseases Treatment Protocol Reactivation Buffer Solution M-E-S Glycerol D-T-T D-D-M Reaction Mixer Tris Hydrochloride Magnesium Chloride Potassium Chloride Sodium Chloride Liposomes Enzyme Reactivation
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