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En roman Stromal Fibroblast-Moduleret 3D Tumor Spheroid Model for at studere Tumor-Stroma Interaktion og Drug Discovery
Journal JoVE
Recherche en cancérologie
This content is Free Access.
Journal JoVE Recherche en cancérologie
A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery
DOI:

07:20 min

February 28, 2020

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Chapitres

  • 00:04Introduction
  • 00:51Melanoma Cell and Skin Fibroblast Culture
  • 01:36Melanoma Cell and Fibroblast Fluorescent Lentivirus Transfection
  • 02:22Melanoma Cell:Fibroblast Co-Culture and Melanoma Cell Solo Culture
  • 03:25Live Cell Time-Lapse Imaging
  • 04:22Confocal Microscopic 3D Movie Generation
  • 04:59Results: Representative 3D Tumor Spheroid Formation and Analysis
  • 06:32Conclusion

Summary

Traduction automatique

En ny tre-dimensionel sfænode model baseret på den heterotypic interaktion af tumorceller og stromale fibroblaster er etableret. Her præsenterer vi coculture af tumorceller og stromale fibroblaster, time-lapse imaging, og konfokal mikroskopi til at visualisere dannelsen af sfæller. Denne tre-dimensionelle model tilbyder en relevant platform til at studere tumor-stroma interaktioner og teste kræft terapeutiske.

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