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Biochemistry
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JoVE Journal Biochemistry
PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays
 

PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays

Article DOI: 10.3791/60661-v • 15:04 min • November 2nd, 2019
November 2nd, 2019 •
1, 2, 1
1Department of Biochemistry, University of British Columbia, 2School of Biochemistry, University of Bristol

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We present a method that combines membrane protein purification and reconstitution into peptidiscs in a single chromatographic step. Biotinylated scaffolds are used for direct surface attachment and measurement of protein-ligand interactions via biolayer interferometry.

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Keywords: Membrane Proteins Peptidisc Biotinylated Peptidiscs Protein Binding Assays Detergent-free Bio-layer Interferometry FhuA Outer Membrane Vesicles PeptiQuick Reconstitution Nickel-NTA IMAC Biotinylated Peptide Streptavidin Coated Sensors
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