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Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

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Génétique

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Journal JoVE Génétique

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

DOI:

10.3791/60697-v

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10:19 min

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February 03, 2020

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Jinxia Shi, Yijuan Jia, Di Fang, Shidan He, Peng Zhang², Yushuang Guo, Yongli Qiao

¹Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences,Shanghai Normal University, ²College of Agriculture,Yangtze University, ³Laboratory of Molecular Genetics, China National Tobacco Corporation,Guizhou Institute of Tobacco Science

Chapitres

00:04Introduction
00:26Preparation of N. benthamiana 16c Plants
02:08Preparation of Agrobacterium Culture for Infiltration
03:44Co-infiltration N. benthamiana Leaves
04:26GFP Imaging Analysis
04:47Northern Blot Analysis of GFP mRNA Levels in Infiltrated Leaves
08:08Results: Infiltrated N. benthamiana 16c Leaves
09:24Conclusion

Summary

Traduction automatique

Here, we present a modified screening method that can be extensively used to quickly screen RNA silencing suppressors in plant pathogens.

Tags

RNA Silencing Suppressors Plant Pathogens Screening Assay Nicotiana Benthamiana Systemic RNA Silencing Local RNA Silencing Agrobacterium LB Medium Antibiotics MES Acetosyringone

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