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Biologie du développement
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Retrograde Tracing of Drosophila Embryonic Motor Neurons Using Lipophilic Fluorescent Dyes
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Biologie du développement
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Journal JoVE Biologie du développement
Retrograde Tracing of Drosophila Embryonic Motor Neurons Using Lipophilic Fluorescent Dyes

Retrograde Tracing of Drosophila Embryonic Motor Neurons Using Lipophilic Fluorescent Dyes

DOI:
10.3791/60716-v

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08:25 min

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January 12, 2020

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, ,
1Department of Cellular Biology,University of Georgia

Chapitres

  • 00:04Titre
  • 00:49Preparation of Equipment
  • 02:03Embryo Staging
  • 03:10Dissection and Staining
  • 04:14Dye Injection and Confocal Microscopy
  • 06:45Results: Retrograde Labeling of aCC and RP3 Motor Neurons
  • 07:32Conclusion

Summary

Traduction automatique

Traduction automatique

We describe a method for retrograde tracing of the Drosophila embryonic motor neurons using lipophilic fluorescent dyes.

Tags

Keywords: DrosophilaEmbryonicMotor NeuronsLipophilicFluorescent DyesRetrograde TracingNeuronal MorphogenesisConnectivityAxon TerminalMicroinjectorDissectionEmbryo CollectingFiltrationMicropipetteBevelingBleachDechorionation

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