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澄清和成像念珠菌生物
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Clarifying and Imaging Candida albicans Biofilms
DOI:

11:09 min

March 06, 2020

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Chapitres

  • 00:04Introduction
  • 00:48Growth of Biofilm Cultures
  • 01:34Specimen Processing for Imaging
  • 02:39Clarification Protocol: Biofilms on Impermeant Substrata
  • 04:30Imaging Setup for Confocal Microscopy
  • 05:41Fast Image Processing and Display for Large Confocal Image Stacks
  • 08:21Results: Optimum Index Matching and Deep Imaging of Mutant and Wild Type C. albicans Biofilms
  • 09:49Conclusion

Summary

Traduction automatique

为了查看和量化念珠菌生物膜的内部特征,我们制备了固定完整的标本,通过折射率匹配进行澄清。然后,利用光学切片显微镜通过生物膜全厚度获取三维图像数据。

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