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Immunologie et infection
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Humaniserede NOG-mus til intravaginal hiv-eksponering og behandling af hiv-infektion
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Immunologie et infection
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Journal JoVE Immunologie et infection
Humanized NOG Mice for Intravaginal HIV Exposure and Treatment of HIV Infection

Humaniserede NOG-mus til intravaginal hiv-eksponering og behandling af hiv-infektion

DOI:
10.3791/60723-v

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08:15 min

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January 31, 2020

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, , , , , , , , ,
1Department of Clinical Medicine,Aarhus University, 2Department of Infectious Diseases,Aarhus University Hospital, 3Department of Biomedicine,Aarhus University, 4Department of Gynecology and Obstetrics,Aarhus University Hospital, 5Department of Biology,University of Nebraska at Omaha

Chapitres

  • 00:04Titre
  • 00:50Cord Blood CCR5Δ32 Genetic Variant Screening
  • 01:50Intravaginal HIV Exposure
  • 03:20RNA Extraction, Complementary DNA (cDNA) Synthesis, and Viral RNA Quantification BY Droplet Digital Polymerase Chain Reaction (ddPCR)
  • 04:55Application of Combination Antiretroviral Therapy (cART)-Containing Chow
  • 05:42Results: Representative HIV infection and Treatment DATA
  • 07:13Conclusion

Summary

Traduction automatique

Traduction automatique

Vi har udviklet en protokol til generering og evaluering af en humaniseret og human immundefekt virus-inficeret NOG musemodel baseret på stamcelletransplantation, intravaginal human immundefekt virus eksponering, og dråbe digital PCR RNA Kvantificering.

Tags

Humanized NOG MiceIntravaginal HIV ExposureHIV InfectionPreclinical HIV ResearchViral StrainsHIV InterventionsCCR5 VariantViral RNA QuantificationCord Blood ScreeningCCR5 Delta 32HIV Exposure Protocol

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