Journal
/
Génétique
/
Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach
Journal JoVE
Génétique
Author Produced
This content is Free Access.
Journal JoVE Génétique
Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

DOI:
10.3791/60749-v

•

12:32 min

•

December 14, 2019

•

, , ,
1Institute of Microbiology,Kiel University

Chapitres

  • 00:00Titre
  • 01:53Monitoring Plasmid-carrying Bacteria under Various Conditions over Time
  • 10:14Résultats
  • 11:22Conclusion

Summary

Traduction automatique

Traduction automatique

Our experimental approach provides a strategy to follow plasmid abundance and antibiotic resistance over time in bacterial populations.

Tags

PlasmidAntibiotic ResistanceLateral Gene TransferPCRIsothermal AssemblyE. ColiMG1655Experimental EvolutionReplica Plating96-well Plate

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection

Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat

QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Producing Gene Deletions in Escherichia coli by P1 Transduction with Excisable Antibiotic Resistance Cassettes

Screening Cotton Genotypes for Reniform Nematode Resistance

A FACS-based Protocol to Isolate RNA from the Secondary Cells of Drosophila Male Accessory Glands

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Read Article