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Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis
Journal JoVE
Médecine
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Journal JoVE Médecine
Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis

Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis

DOI:
10.3791/60776-v

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07:40 min

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March 20, 2021

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, , , , , , ,
1Translational Medicine, R&D Oncology,AstraZeneca, 2Acerta Pharma, AstraZeneca Group, 3Translational Medicine, R&D Oncology,AstraZeneca

Chapitres

  • 00:05Introduction
  • 01:08Whole Blood Irradiation for DNA Damage Response (DDR) Biomarker Expression Analysis
  • 03:35PBMC and Plasma Freezing
  • 04:45Western Blot Analysis
  • 05:43Results: Representative DNA Damage Assessment
  • 07:10Conclusion

Summary

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This protocol details clinically implementable preparation of high quality PBMC and plasma biosamples at the clinical trial site that can be used for translational biomarker analysis.

Tags

Keywords: PBMCPlasmaBlood DrawBiomarker AnalysisClinical TrialPre-analytical VariabilityPharmacokineticsPharmacodynamicsDNA Damage ResponseRadiationCentrifugationCell SeparationBuffy Coat

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