Journal
/
Médecine
/
Effect of Artificial Tear Formulations on the Metabolic Activity of Human Corneal Epithelial Cells after Exposure to Desiccation
Journal JoVE
Médecine
This content is Free Access.
Journal JoVE Médecine
Effect of Artificial Tear Formulations on the Metabolic Activity of Human Corneal Epithelial Cells after Exposure to Desiccation

Effect of Artificial Tear Formulations on the Metabolic Activity of Human Corneal Epithelial Cells after Exposure to Desiccation

DOI:
10.3791/60812-v

•

06:29 min

•

May 02, 2020

•

, , , , , , , ,
1Alcon Vision, LLC, 2Centre for Ocular Research and Education (CORE), School of Optometry & Vision Science,University of Waterloo

Chapitres

  • 00:05Introduction
  • 00:52Cell Preparation
  • 02:26No Desiccation Protocol
  • 03:41Desiccation Protocol
  • 04:54Results: Effect of Dry Eye Lipid Enhanced Products on Cell Viability and Desiccation Protection
  • 05:42Conclusion

Summary

Traduction automatique

Traduction automatique

The purpose of this protocol is to evaluate if different artificial tear formulations can protect human corneal epithelial cells from desiccation using an in vitro model. After exposure to artificial tear formulations and desiccation, human corneal epithelial cells are assessed for metabolic activity.

Tags

Keywords: Corneal Epithelial CellsDesiccationArtificial Tear FormulationsMetabolic ActivityCell CultureCell Viability AssayFluorescence Measurement

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/60812?article_type=v

Isolation of Mouse Respiratory Epithelial Cells and Exposure to Experimental Cigarette Smoke at Air Liquid Interface
check_url/fr/60812?article_type=v

Formation of Human Prostate Epithelium Using Tissue Recombination of Rodent Urogenital Sinus Mesenchyme and Human Stem Cells
check_url/fr/60812?article_type=v

Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications
check_url/fr/60812?article_type=v

Systems Biology of Metabolic Regulation by Estrogen Receptor Signaling in Breast Cancer
check_url/fr/60812?article_type=v

Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation
check_url/fr/60812?article_type=v

Measuring the Effect of Chemicals on the Growth and Reproduction of Caenorhabditis elegans
check_url/fr/60812?article_type=v

A Primary Human Trophoblast Model to Study the Effect of Inflammation Associated with Maternal Obesity on Regulation of Autophagy in the Placenta
check_url/fr/60812?article_type=v

A Murine Model of Fetal Exposure to Maternal Inflammation to Study the Effects of Acute Chorioamnionitis on Newborn Intestinal Development
check_url/fr/60812?article_type=v

Electroporation-Based Genetic Modification of Primary Human Pigment Epithelial Cells Using the Sleeping Beauty Transposon System
check_url/fr/60812?article_type=v

An Epithelial Abrasion Model for Studying Corneal Wound Healing

Read Article