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Synthesis of Functionalized Magnetic Nanoparticles, Their Conjugation with the Siderophore Feroxamine and its Evaluation for Bacteria Detection
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Journal JoVE Chimie
Synthesis of Functionalized Magnetic Nanoparticles, Their Conjugation with the Siderophore Feroxamine and its Evaluation for Bacteria Detection

Synthesis of Functionalized Magnetic Nanoparticles, Their Conjugation with the Siderophore Feroxamine and its Evaluation for Bacteria Detection

DOI:
10.3791/60842-v

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15:03 min

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June 16, 2020

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, , , , , ,
1Centro de Investigacións Científicas Avanzadas (CICA), Departamento de Química, Facultade de Ciencias,Universidade da Coruña, 2Centro de Investigacións Científicas Avanzadas (CICA), Unidad de Comunicación y Divulgación,Universidade da Coruña, 3Department of Microbiology and Parasitology, Institute of Aquaculture,Universidade de Santiago de Compostela

Chapitres

  • 00:00Introduction
  • 01:06Synthesis of Magnetic Nanoparticles Conjugated with Feroxamine
  • 09:57Bacterial Assay with Y. ENTEROCOLITICA Strains to Quantify the Isolation and Capture of Pathogenic Bacteria with Nanoparticles
  • 13:52Résultats

Summary

Traduction automatique

Traduction automatique

This work describes protocols for the preparation of magnetic nanoparticles, its coating with SiO2, followed by its amine functionalization with (3-aminopropyl)triethoxysilane (APTES) and its conjugation with deferoxamine using a succinyl moiety as a linker. A deep structural characterization description and a capture bacteria assay using Y. enterocolitica for all the intermediate nanoparticles and the final conjugate are also described in detail.

Tags

Keywords: Magnetic NanoparticlesSilica CoatingAmine FunctionalizationSiderophore FeroxamineBacteria DetectionIron UptakeNanoparticle SynthesisCore-shell StructureSurface ModificationAnalytical ScienceNanomedicine

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