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Morphological and Functional Evaluation of Axons and their Synapses during Axon Death in Drosophila melanogaster
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Morphological and Functional Evaluation of Axons and their Synapses during Axon Death in Drosophila melanogaster

Morphological and Functional Evaluation of Axons and their Synapses during Axon Death in Drosophila melanogaster

DOI:
10.3791/60865-v

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10:29 min

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March 16, 2020

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, , ,
1Department of Fundamental Neurosciences,University of Lausanne

Chapitres

  • 00:05Introduction
  • 01:28Observation of Axon Morphology During Axon Death in the PNS (Peripheral Nervous System)
  • 03:22Observation of Axon and Synapse Morphology During Axon Death in the CNS (Central Nervous System)
  • 05:53Grooming Induced by Optogenetics as a Readout for Axon and Synapse Function
  • 07:56Results: Axon Death of GFP-Labeled Sensory Neuron Axons and Axonal/Synaptic Function After Axotomy
  • 09:40Conclusion

Summary

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Here, we provide protocols to perform three simple injury-induced axon degeneration (axon death) assays in Drosophila melanogaster to evaluate the morphological and functional preservation of severed axons and their synapses.

Tags

Drosophila MelanogasterAxon DegenerationAxon MorphologySynapse FunctionPartial Wing InjuryAntennal AblationOptogeneticsNeuronal Maintenance FactorsAxonal TransportAxonal OrganellesSpinning Disk Microscopy

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