Opsono-Adherence Assay to Evaluate Functional Antibodies in Vaccine Development Against <em>Bacillus anthracis</em> and Other Encapsulated Pathogens

Jennifer Chua; Donald  J. Chabot; Tammy Putmon-Taylor; Arthur  M. Friedlander

doi:10.3791/60873

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Opsono-overholdelse analyse for å evaluere funksjonelle antistoffer i vaksineutvikling mot Bacillus anthracis og andre innkapslede patogener

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Opsono-Adherence Assay to Evaluate Functional Antibodies in Vaccine Development Against Bacillus anthracis and Other Encapsulated Pathogens

Opsono-overholdelse analyse for å evaluere funksjonelle antistoffer i vaksineutvikling mot Bacillus anthracis og andre innkapslede patogener

DOI:

10.3791/60873-v

•

13:47 min

•

May 19, 2020

•

Jennifer Chua*¹, Donald J. Chabot*¹, Tammy Putmon-Taylor³, Arthur M. Friedlander

¹Bacteriology Division,United States Army Medical Research Institute of Infectious Diseases, ²Headquarters Division,United States Army Medical Research Institute of Infectious Diseases, ³Bioqual

Chapters

00:04Introduction
01:21Plating of the Cell Line, RAW 264.7 and Bacterial Culture
06:29FITC-labeling of Bacteria and Bacterial Opsonization
08:46Adherence Assay and Fluorescent Labeling of Mammalian Cells
10:22Microscopy
11:33Results: Representative Micrographs Collected During an OAA Experiment to Evaluate the Efficacy of an Anthrax Vaccine
13:01Conclusion

Summary

Automatic Translation

Opsono-adherence analysen er en alternativ metode for opsono-fagocytisk drapsanalyse for å evaluere opsonisk funksjoner av antistoffer i vaksineutvikling.

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