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Fabricating Multi-Component Lipid Nanotube Networks Using the Gliding Kinesin Motility Assay
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Bio-ingénierie
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Journal JoVE Bio-ingénierie
Fabricating Multi-Component Lipid Nanotube Networks Using the Gliding Kinesin Motility Assay

Fabricating Multi-Component Lipid Nanotube Networks Using the Gliding Kinesin Motility Assay

DOI:
10.3791/60899-v

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05:16 min

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July 26, 2021

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1Center for Integrated Nanotechnologies,Sandia National Laboratories

Chapitres

  • 00:04Introduction
  • 00:32Gliding Motility Assay (GMA)
  • 01:55Lipid Nanotube (LNT) Network Imaging
  • 02:31LNT Network Characterization
  • 03:41Results: Representative LNTs Fabricated from Different GUV Lipid Formulations
  • 04:36Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol describes a process for fabricating lipid nanotube networks using gliding kinesin motility in conjunction with giant unilamellar lipid vesicles.

Tags

Lipid NanotubeGliding Motility AssayKinesinMicrotubuleStreptavidinGUVAMP-PNPImage AnalysisNanotube LengthLipid Partitioning

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