Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Medicine / Slicing and Culturing Pig Hearts under…
JoVE Journal
Medicine

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

JoVE Journal Medicine
Slicing and Culturing Pig Hearts under Physiological Conditions
 

Slicing and Culturing Pig Hearts under Physiological Conditions

Article DOI: 10.3791/60913-v • 07:54 min • March 20th, 2020
March 20th, 2020 •
*1, *1, 1, 1, 2, 2, 2, 2, 1, 1,3,4,5,6
1Institute of Molecular Cardiology, Department of Medicine, University of Louisville, 2Department of Bioengineering, University of Louisville, 3Diabetes and Obesity Center, Department of Medicine, University of Louisville, 4Department of Pharmacology and Toxicology, University of Louisville, 5Institute of Cardiovascular Sciences, University of Manchester, 6Faculty of Pharmacy, Zagazig University
* These authors contributed equally

Chapitres

résumé
Automatic Translation

This protocol describes how to slice and culture heart tissue under physiological conditions for 6 days. This culture system could be used as a platform for testing the efficacy of novel heart failure therapeutics as well as reliable testing of acute cardiotoxicity in a 3D heart model.

Tags

Keywords: Pig Heart Heart Tissue Slicing Culturing Physiological Conditions Acute Cardiac Toxicity Heart Failure Therapy Vibratome Tyrode Solution Cardioplegic Solution Tissue Glue Agar Cell Strainer
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter